Some Aspects of Carbohydrate Metabolism and Production of Glycosyltransferases from Mutant Strain Leuconostoc Mesenteroides M2860
نویسندگان
چکیده
The production of glycosyltransferases (GTFs) from constitutive mutant strain Leuconostoc mesenteroides M2860 was studied. When grown in glucose medium in the absence of sucrose Leuc. mesenteroides M2860 produced low, but detectable GTF activity. Much of the GTF activity (81%) in sucrose grown cultures was located in the cell pellets. Extracellular and cell associated activities were determined when run on SDS-PAGE for in situ activity detection by periodic acid-Schiff s staining. In situ analysis showed single band corresponding to 180 kDa molecular size in supernatant and enzyme concentrate, received by cultivation on glucose media. The enzyme concentrate and supernatant fraction obtained by fermentation on sucrose media showed three bands corresponding to 180 kDa, 120 kDa and 86 kDa.
منابع مشابه
UV Mutagenesis of Leuconostoc mesenteroides NRRL B-640 for Generation of a Mutant (B-640M) with Hyper-Producing Dextransucrase Activity
This study reports the strain improvement of Leuconostoc mesenteroides NRRL B-640 by classical mutation technique using ultraviolet radiation to achieve the mutant strain with higher dextransucrase activity. A large number of mutants were generated, cultured and tested for dextransucrase activity. The selected mutant gave considerably higher (46%) dextransucrase activity (0.8 U/mg) as compared ...
متن کاملInfluence of terpenoids and acarbose on glycosyltransferases produced by strain Leuconostoc mesenteroides URE 13
A study of the influence of different plant terpenoids and amino sugar derivate acarbose on the activity of glycosyltransferase complex and purified dextransucrase from Leuconostoc mesenteroides URE 13 strain was carried out. All the tested terpenoids showed an inhibitory effect on glycosyltransferases from strain URE 13 at concentration 0.34 mmol. Out of all studied diterpenoids splendidin sho...
متن کاملComparison of exopolysaccharide production by indigenous Leuconostoc mesenteroides strains in whey medium
Two Leuconostoc mesenteroides strains NCDC 744 and NCDC 745 were evaluated for exopolysaccharides (EPS) production in whey and MRS medium. Paneer whey medium supplemented with 10% sucrose, 0.1 % yeast extract and 0.1 % K2HPO4 was found to be proferred medium for EPS production. Among two strains L. mesenteroides NCDC 744 produced significantly higher EPS (12.7 ± 0.24 gm/L) as compared to NCDC 7...
متن کاملProton motive force generation by citrolactic fermentation in Leuconostoc mesenteroides.
In Leuconostoc mesenteroides subsp. mesenteroides 19D, citrate is transported by a secondary citrate carrier (CitP). Previous studies of the kinetics and mechanism of CitP performed in membrane vesicles of L. mesenteroides showed that CitP catalyzes divalent citrate HCit2-/H+ symport, indicative of metabolic energy generation by citrate metabolism via a secondary mechanism (C. Marty-Teysset, J....
متن کاملCharacterization of the Dextran Produced by Leuconostoc mesenteroides from Date Fruit Extract
In the present study, the production of dextran from date extract and sucrose as carbon sources by the bacterium Leuconostoc mesenteroides NRRL B512 (f) was investigated. In comparison to blue dextran (Mw≈2000 Da), dextran molecular weight was reduced (Mw2000 KDa). Flow behavior indices of dextran solutions from date extracts were investigated and rheological parameters were evaluated at concen...
متن کامل